diff --git a/client/ayon_core/hosts/maya/api/plugin.py b/client/ayon_core/hosts/maya/api/plugin.py
index 2a1a531012..c5e3f42d10 100644
--- a/client/ayon_core/hosts/maya/api/plugin.py
+++ b/client/ayon_core/hosts/maya/api/plugin.py
@@ -925,8 +925,8 @@ class ReferenceLoader(Loader):
     def prepare_root_value(self, file_url, project_name):
         """Replace root value with env var placeholder.
 
-        Use ${OPENPYPE_ROOT_WORK} (or any other root) instead of proper root
-        value when storing referenced url into a workfile.
+        Use ${AYON_PROJECT_ROOT_WORK} (or any other root) instead of proper
+        root value when storing referenced url into a workfile.
         Useful for remote workflows with SiteSync.
 
         Args:
diff --git a/client/ayon_core/hosts/nuke/api/lib.py b/client/ayon_core/hosts/nuke/api/lib.py
index eb85c14fdb..86496a4804 100644
--- a/client/ayon_core/hosts/nuke/api/lib.py
+++ b/client/ayon_core/hosts/nuke/api/lib.py
@@ -2227,7 +2227,7 @@ Reopening Nuke should synchronize these paths and resolve any discrepancies.
 
         # include all project root related env vars
         for env_var in os.environ:
-            if env_var.startswith("OPENPYPE_PROJECT_ROOT_"):
+            if env_var.startswith("AYON_PROJECT_ROOT_"):
                 included_vars.append(env_var)
 
         # use regex to find env var in template with format {ENV_VAR}
diff --git a/client/ayon_core/pipeline/anatomy.py b/client/ayon_core/pipeline/anatomy.py
index 777158edd1..86b7d92309 100644
--- a/client/ayon_core/pipeline/anatomy.py
+++ b/client/ayon_core/pipeline/anatomy.py
@@ -125,7 +125,7 @@ class BaseAnatomy(object):
         return self._roots_obj
 
     def root_environments(self):
-        """Return OPENPYPE_ROOT_* environments for current project in dict."""
+        """Return AYON_PROJECT_ROOT_* environments for current project."""
         return self._roots_obj.root_environments()
 
     def root_environmets_fill_data(self, template=None):
@@ -150,7 +150,7 @@ class BaseAnatomy(object):
         return self.roots_obj.all_root_paths()
 
     def set_root_environments(self):
-        """Set OPENPYPE_ROOT_* environments for current project."""
+        """Set AYON_PROJECT_ROOT_* environments for current project."""
         self._roots_obj.set_root_environments()
 
     def root_names(self):
@@ -289,7 +289,7 @@ class BaseAnatomy(object):
         "<{}>"
 
         ## Output
-        "<OPENPYPE_PROJECT_ROOT_NAS>/project/asset/task/animation_v001.ma"
+        "<AYON_PROJECT_ROOT_NAS>/project/asset/task/animation_v001.ma"
 
         Args:
             filepath (str): Full file path where root should be replaced.
@@ -1206,7 +1206,7 @@ class Roots:
         anatomy Anatomy: Anatomy object created for a specific project.
     """
 
-    env_prefix = "OPENPYPE_PROJECT_ROOT"
+    env_prefix = "AYON_PROJECT_ROOT"
     roots_filename = "roots.json"
 
     def __init__(self, anatomy):
@@ -1312,8 +1312,8 @@ class Roots:
     def root_environments(self):
         """Use root keys to create unique keys for environment variables.
 
-        Concatenates prefix "OPENPYPE_ROOT" with root keys to create unique
-        keys.
+        Concatenates prefix "AYON_PROJECT_ROOT_" with root keys to create
+        unique keys.
 
         Returns:
             dict: Result is `{(str): (str)}` dicitonary where key represents
@@ -1335,14 +1335,10 @@ class Roots:
 
             Result on windows platform::
                 {
-                    "OPENPYPE_ROOT_WORK": "P:/projects/work",
-                    "OPENPYPE_ROOT_PUBLISH": "P:/projects/publish"
+                    "AYON_PROJECT_ROOT_WORK": "P:/projects/work",
+                    "AYON_PROJECT_ROOT_PUBLISH": "P:/projects/publish"
                 }
 
-            Short example when multiroot is not used::
-                {
-                    "OPENPYPE_ROOT": "P:/projects"
-                }
         """
         return self._root_environments()