diff --git a/client/ayon_core/hosts/nuke/plugins/publish/collect_slate_node.py b/client/ayon_core/hosts/nuke/plugins/publish/collect_slate_node.py
index 3baa0cd9b5..ac30bd6051 100644
--- a/client/ayon_core/hosts/nuke/plugins/publish/collect_slate_node.py
+++ b/client/ayon_core/hosts/nuke/plugins/publish/collect_slate_node.py
@@ -17,7 +17,8 @@ class CollectSlate(pyblish.api.InstancePlugin):
             (
                 n_ for n_ in nuke.allNodes()
                 if "slate" in n_.name().lower()
-                if not n_["disable"].getValue()
+                if not n_["disable"].getValue() and
+                "publish_instance" not in n_.knobs()  # Exclude instance nodes.
             ),
             None
         )