diff --git a/openpype/lib/file_transaction.py b/openpype/lib/file_transaction.py
index fe70b37cb1..81332a8891 100644
--- a/openpype/lib/file_transaction.py
+++ b/openpype/lib/file_transaction.py
@@ -13,6 +13,16 @@ else:
     from shutil import copyfile
 
 
+class DuplicateDestinationError(ValueError):
+    """Error raised when transfer destination already exists in queue.
+
+    The error is only raised if `allow_queue_replacements` is False on the
+    FileTransaction instance and the added file to transfer is of a different
+    src file than the one already detected in the queue.
+
+    """
+
+
 class FileTransaction(object):
     """File transaction with rollback options.
 
@@ -44,7 +54,7 @@ class FileTransaction(object):
     MODE_COPY = 0
     MODE_HARDLINK = 1
 
-    def __init__(self, log=None):
+    def __init__(self, log=None, allow_queue_replacements=False):
         if log is None:
             log = logging.getLogger("FileTransaction")
 
@@ -60,6 +70,8 @@ class FileTransaction(object):
         # Backup file location mapping to original locations
         self._backup_to_original = {}
 
+        self._allow_queue_replacements = allow_queue_replacements
+
     def add(self, src, dst, mode=MODE_COPY):
         """Add a new file to transfer queue.
 
@@ -82,6 +94,14 @@ class FileTransaction(object):
                         src, dst))
                 return
             else:
+                if not self._allow_queue_replacements:
+                    raise DuplicateDestinationError(
+                        "Transfer to destination is already in queue: "
+                        "{} -> {}. It's not allowed to be replaced by "
+                        "a new transfer from {}".format(
+                            queued_src, dst, src
+                        ))
+
                 self.log.warning("File transfer in queue replaced..")
                 self.log.debug(
                     "Removed from queue: {} -> {} replaced by {} -> {}".format(
diff --git a/openpype/plugins/publish/integrate.py b/openpype/plugins/publish/integrate.py
index 6a0327ec84..760b1a6b37 100644
--- a/openpype/plugins/publish/integrate.py
+++ b/openpype/plugins/publish/integrate.py
@@ -24,7 +24,10 @@ from openpype.client import (
     get_version_by_name,
 )
 from openpype.lib import source_hash
-from openpype.lib.file_transaction import FileTransaction
+from openpype.lib.file_transaction import (
+    FileTransaction,
+    DuplicateDestinationError
+)
 from openpype.pipeline.publish import (
     KnownPublishError,
     get_publish_template_name,
@@ -170,9 +173,18 @@ class IntegrateAsset(pyblish.api.InstancePlugin):
             ).format(instance.data["family"]))
             return
 
-        file_transactions = FileTransaction(log=self.log)
+        file_transactions = FileTransaction(log=self.log,
+                                            # Enforce unique transfers
+                                            allow_queue_replacements=False)
         try:
             self.register(instance, file_transactions, filtered_repres)
+        except DuplicateDestinationError as exc:
+            # Raise DuplicateDestinationError as KnownPublishError
+            # and rollback the transactions
+            file_transactions.rollback()
+            six.reraise(KnownPublishError,
+                        KnownPublishError(exc),
+                        sys.exc_info()[2])
         except Exception:
             # clean destination
             # todo: preferably we'd also rollback *any* changes to the database