diff --git a/client/ayon_core/hooks/pre_global_host_data.py b/client/ayon_core/hooks/pre_global_host_data.py
index 12da6f12f8..23f725901c 100644
--- a/client/ayon_core/hooks/pre_global_host_data.py
+++ b/client/ayon_core/hooks/pre_global_host_data.py
@@ -1,12 +1,15 @@
 from ayon_api import get_project, get_folder_by_path, get_task_by_name
 
+from ayon_core.pipeline import Anatomy
+from ayon_core.pipeline.anatomy import RootMissingEnv
+
 from ayon_applications import PreLaunchHook
+from ayon_applications.exceptions import ApplicationLaunchFailed
 from ayon_applications.utils import (
     EnvironmentPrepData,
     prepare_app_environments,
     prepare_context_environments
 )
-from ayon_core.pipeline import Anatomy
 
 
 class GlobalHostDataHook(PreLaunchHook):
@@ -67,9 +70,12 @@ class GlobalHostDataHook(PreLaunchHook):
         self.data["project_entity"] = project_entity
 
         # Anatomy
-        self.data["anatomy"] = Anatomy(
-            project_name, project_entity=project_entity
-        )
+        try:
+            self.data["anatomy"] = Anatomy(
+                project_name, project_entity=project_entity
+            )
+        except RootMissingEnv as exc:
+            raise ApplicationLaunchFailed(str(exc))
 
         folder_path = self.data.get("folder_path")
         if not folder_path:
diff --git a/client/ayon_core/pipeline/anatomy/__init__.py b/client/ayon_core/pipeline/anatomy/__init__.py
index 336d09ccaa..7000f51495 100644
--- a/client/ayon_core/pipeline/anatomy/__init__.py
+++ b/client/ayon_core/pipeline/anatomy/__init__.py
@@ -1,5 +1,6 @@
 from .exceptions import (
     ProjectNotSet,
+    RootMissingEnv,
     RootCombinationError,
     TemplateMissingKey,
     AnatomyTemplateUnsolved,
@@ -9,6 +10,7 @@ from .anatomy import Anatomy
 
 __all__ = (
     "ProjectNotSet",
+    "RootMissingEnv",
     "RootCombinationError",
     "TemplateMissingKey",
     "AnatomyTemplateUnsolved",
diff --git a/client/ayon_core/pipeline/anatomy/exceptions.py b/client/ayon_core/pipeline/anatomy/exceptions.py
index 39f116baf0..24df0e3046 100644
--- a/client/ayon_core/pipeline/anatomy/exceptions.py
+++ b/client/ayon_core/pipeline/anatomy/exceptions.py
@@ -5,6 +5,11 @@ class ProjectNotSet(Exception):
     """Exception raised when is created Anatomy without project name."""
 
 
+class RootMissingEnv(KeyError):
+    """Raised when root requires environment variables which is not filled."""
+    pass
+
+
 class RootCombinationError(Exception):
     """This exception is raised when templates has combined root types."""
 
diff --git a/client/ayon_core/pipeline/anatomy/roots.py b/client/ayon_core/pipeline/anatomy/roots.py
index 2773559d49..bd09a9fe51 100644
--- a/client/ayon_core/pipeline/anatomy/roots.py
+++ b/client/ayon_core/pipeline/anatomy/roots.py
@@ -2,9 +2,11 @@ import os
 import platform
 import numbers
 
-from ayon_core.lib import Logger
+from ayon_core.lib import Logger, StringTemplate
 from ayon_core.lib.path_templates import FormatObject
 
+from .exceptions import RootMissingEnv
+
 
 class RootItem(FormatObject):
     """Represents one item or roots.
@@ -21,18 +23,36 @@ class RootItem(FormatObject):
             multi root setup otherwise None value is expected.
     """
     def __init__(self, parent, root_raw_data, name):
-        super(RootItem, self).__init__()
+        super().__init__()
         self._log = None
-        lowered_platform_keys = {}
-        for key, value in root_raw_data.items():
-            lowered_platform_keys[key.lower()] = value
+        lowered_platform_keys = {
+            key.lower(): value
+            for key, value in root_raw_data.items()
+        }
         self.raw_data = lowered_platform_keys
         self.cleaned_data = self._clean_roots(lowered_platform_keys)
         self.name = name
         self.parent = parent
 
         self.available_platforms = set(lowered_platform_keys.keys())
-        self.value = lowered_platform_keys.get(platform.system().lower())
+
+        current_platform = platform.system().lower()
+        # WARNING: Using environment variables in roots is not considered
+        #   as production safe. Some features may not work as expected, for
+        #   example USD resolver or site sync.
+        try:
+            self.value = lowered_platform_keys[current_platform].format_map(
+                os.environ
+            )
+        except KeyError:
+            result = StringTemplate(self.value).format(os.environ.copy())
+            is_are = "is" if len(result.missing_keys) == 1 else "are"
+            missing_keys = ", ".join(result.missing_keys)
+            raise RootMissingEnv(
+                f"Root \"{name}\" requires environment variable/s"
+                f" {missing_keys} which {is_are} not available."
+            )
+
         self.clean_value = self._clean_root(self.value)
 
     def __format__(self, *args, **kwargs):
@@ -105,10 +125,10 @@ class RootItem(FormatObject):
 
     def _clean_roots(self, raw_data):
         """Clean all values of raw root item values."""
-        cleaned = {}
-        for key, value in raw_data.items():
-            cleaned[key] = self._clean_root(value)
-        return cleaned
+        return {
+            key: self._clean_root(value)
+            for key, value in raw_data.items()
+        }
 
     def path_remapper(self, path, dst_platform=None, src_platform=None):
         """Remap path for specific platform.