diff --git a/client/ayon_core/pipeline/traits/content.py b/client/ayon_core/pipeline/traits/content.py
index 0ca0cbb3e1..4d198813c1 100644
--- a/client/ayon_core/pipeline/traits/content.py
+++ b/client/ayon_core/pipeline/traits/content.py
@@ -140,7 +140,7 @@ class FileLocations(TraitBase):
             Optional[FileLocation]: File location for the frame.
 
         """
-        frame_regex = r"\.(?P<frame>(?P<padding>0*)\d+)\.\D+\d?$"
+        frame_regex = r"\.(?P<index>(?P<padding>0*)\d+)\.\D+\d?$"
         if sequence_trait and sequence_trait.frame_regex:
             frame_regex = sequence_trait.frame_regex
 
@@ -148,7 +148,7 @@ class FileLocations(TraitBase):
         for location in self.file_paths:
             result = re.search(frame_regex, location.file_path.name)
             if result:
-                frame_index = int(result.group("frame"))
+                frame_index = int(result.group("index"))
                 if frame_index == frame:
                     return location
         return None
diff --git a/client/ayon_core/pipeline/traits/time.py b/client/ayon_core/pipeline/traits/time.py
index 74da6ef570..7687a023da 100644
--- a/client/ayon_core/pipeline/traits/time.py
+++ b/client/ayon_core/pipeline/traits/time.py
@@ -118,7 +118,8 @@ class Sequence(TraitBase):
             sequence.
         frame_padding (int): Frame padding.
         frame_regex (str): Frame regex - regular expression to match
-            frame numbers. Must include 'frame' named group.
+            frame numbers. Must include 'index' named group and 'padding'
+            named group.
         frame_spec (str): Frame list specification of frames. This takes
             string like "1-10,20-30,40-50" etc.
 
@@ -136,8 +137,8 @@ class Sequence(TraitBase):
     @classmethod
     def validate_frame_regex(cls, v: Optional[str]) -> str:
         """Validate frame regex."""
-        if v is not None and "?P<frame>" not in v:
-            msg = "Frame regex must include 'frame' named group"
+        if v and any(s not in v for s in ["?P<index>", "?P<padding>"]):
+            msg = "Frame regex must include 'index' and `padding named groups"
             raise ValueError(msg)
         return v